Hyperspectral Imaging of Fluorescent Dyes

Resonon utilizes line-scan spectral imaging, where a scene is limited to one dimension with a slit, and then imaged off a diffraction grating to obtain spectral information for each pixel along the slit. A second spatial dimension is obtained by scanning the object as multiple frames are collected. Here, electrophoresis was used as the scanning mechanism, as illustrated in Figures 1 and 2. Proteins tagged with fluorescent dyes self-scanned as they migrated through the gel. This approach eliminated all moving parts, eliminated the need for a second scanning process after electrophoresis, and produced excellent signal-to-noise due to long integration times.